Process and apparatus for conversion of atz to pth amino acid de

Details Process and apparatus for conversion of atz to pth amino acid de Process and apparatus for conversion of atz to pth amino acid de

1978-03-31

1979-05-22

Serwin, R. E.

Chemistry: physical processes

Physical processes

Crystallization

23230A, 2601125R, 422109, C07C10352

Patent

active

041557140

ABSTRACT:
A process and apparatus for the conversion of anilinothiazolino amino acid derivatives of peptides and proteins for use with conventional sequencers in the overall Edman degradation process. The apparatus comprises a modular unit separate from the sequencer having an independently programmable control and flow actuating and directing means including a source of pressurized nitrogen, a conversion reagent container, a wash solvent container, a reaction vessel, and a plurality of interconnecting tubes and valves controlled so as to regulate the amounts and durations of all fluids entering and leaving said reaction vessel under gas pressure.
The process comprises performing the steps of conversion, sample recovery, drying and washing at temperatures below but near to the respective boiling points of both the reagent and the solvent, and under reflux tower conditions. A specific embodiment employs anhydrous methanolic hydrogen chloride for the reagent and dichloroethane methanol (7:3) for the solvent, and both the conversion and the washing are done at 65.degree. C., in a reaction vessel comprising a lower, heated portion, and an upper, cooled portion where the reflux action takes place both during the conversion and washing steps.

REFERENCES:
patent: 3531258 (1970-09-01), Merrifield et al.
patent: 3557077 (1971-01-01), Brunfeldt et al.
patent: 3647390 (1972-03-01), Kubodera et al.
patent: 3725010 (1973-04-01), Penhasi
patent: 3951741 (1976-04-01), Pfaender
patent: 4065412 (1977-12-01), Dreyer

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