Process and apparatus for ascertaining the concentration of micr

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23253R, 23259, 195127, 195139, C12K 104

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040186520

ABSTRACT:
The concentration of microorganisms in a known volume of a water specimen is ascertained by introducing the water specimen into a plurality of wells which have known volume and contain a nutrient medium. The mixture of water specimen and nutrient medium is incubated and the wells are observed for a change in the appearance thereof which indicates metabolic activity, that is, the existence of microorganisms in the wells. If all the wells change appearance, then it is known that the concentration exceeds a certain limit, that is, at least one cell per specific well volume. On the other hand, if none of the wells change, then it is most likely the concentration is less than one cell per total volume of specimen in the wells. A change in appearance of some but not all of the wells indicates a concentration between the foregoing limits, and this concentration is estimated by statistical evaluation based on proven statistical computations.

REFERENCES:
patent: 3957583 (1976-05-01), Gibson et al.
Martin Frobisher, Fundamentals of Microbiology, 8th Edition, W. B. Saunders Company, 1968, pp. 46-49.

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