Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1977-11-25
1980-06-10
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
422 61, 435300, 435301, 435287, 435291, C12Q 104, C12M 118
Patent
active
042073944
ABSTRACT:
Microorganisms in a specimen are detected, identified, and enumerated by introducing the specimen into a sampling cartridge and diluting the specimen with a known volume of water within the cartridge. The cartridge has a manifold and several cassettes attached to the manifold. Each cassette contains a serpentine flow channel having a series of filters therein and a detection cell located downstream from each filter. The flow channel in each cassette also contains a culture medium which is freeze dried and is highly selective in the sense that it promotes the growth of one type of microorganism, but not others. The mixture of the specimen and water flows from the manifold into the flow channel of each cassette where it rehydrates the culture medium therein and further flows through the filters. Each filter removes a known proportion of the microorganisms from the mixture of specimen, water and medium, thereby effecting a serial dilution. After the cassettes are heated to incubate the microoganisms, the detection cells are observed for growth of the microorganisms therein which is manifested in a change in the light transmitting characteristics of the mixtures within the cells.
REFERENCES:
patent: 3691017 (1972-09-01), Brown et al.
patent: 3799742 (1974-03-01), Coleman
patent: 3922203 (1975-11-01), Aldridge, Jr. et al.
patent: 3963355 (1976-06-01), Aldridge, Jr. et al.
Robert Bailey and Edwyn Scott, Diagnostic Microbiology; 2nd Edition, The C.V. Mosby Company; pp. 295 and 296; 1966.
Martin Frobisher, Fundamentals of Microbiology, 8th Edition, W. B. Saunders Company, p. 47; 1968.
Aldridge, Jr. Clifton
Gibson Sandra F.
Holen James T.
Jones Paul W.
Keyser George F.
McDonnell Douglas Corporation
Tanenholtz Alvin E.
Warden Robert J.
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