Process and a reagent kit for the determination of direct and to

Details Process and a reagent kit for the determination of direct and to Process and a reagent kit for the determination of direct and to

1983-07-01

1990-01-09

Hill, Jr., Robert J.

Chemistry: analytical and immunological testing

Heterocyclic carbon compound

Hetero-n

422 61, 436903, C01N 3372

Patent

active

048928330

ABSTRACT:
A process and reagent kit for the determination of direct and total bilirubin in body fluids by coupling the bilirubin with a diazonium salt and determining the extinction change thereby brought about, by usual methods.
The coupling is carried out in the presence of a buffer which adjusts the test solution to a weakly acidic to approximately neutral pH value preferably between pH 3.5 and 8. The diazonium salt is of the formula: ##STR1## in which R.sub.1 is a hydrogen or halogen atom or a lower alkyl, lower alkoxy or benzoylamino radical, R.sub.2 is a hydrogen atom or a lower alkyl or lower alkoxy radical, R.sub.3 is a hydrogen or halogen atom or a lower alkyl or lower alkoxy radical, whereby R.sub.1 and R.sub.3 do not simultaneously represent halogen atoms, and X is an anion forming a storage-stable salt with the diazonium cation.

REFERENCES:
patent: 3511607 (1970-05-01), Green
patent: 3585004 (1971-06-01), Mast
patent: 3814586 (1974-06-01), Fraser, Jr. et al.
patent: 3825411 (1974-07-01), Morin
patent: 4468467 (1984-08-01), Babb et al.
Hillman, Z. Klin. Chem. u. Klin. Biochem. 9, Jg., S. 273-274, Mai 1971.
Gedigk et al., Hoppe-Seylers Z. Physiol. Chem. 289, 261-271 (1952).
Gedigk et al., Chemical Abstracts, vol. 48, No. 18, No. 10815e, 1954.

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