Process and a reagent for the simultaneous determination of fibr

Details Process and a reagent for the simultaneous determination of fibr Process and a reagent for the simultaneous determination of fibr

1984-08-20

1987-09-08

Nucker, Christine M.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving blood clotting factor

435 4, 435 23, 435810, 436 69, C12Q 156

Patent

active

046924060

ABSTRACT:
The present invention provides a process for the simultaneous determination of fibrinogen and fibrinogen fission products in plasma, wherein 0.01 to 1 U of a snake venom enzyme with a thrombin-like effectiveness is aded per ml. of sample plasma, then there is measured the period of time between the addition of the enzyme and the commencement of a turbidity formation as a measure for the amount of fibrinogen fission products and subsequently there is measured the speed of the turbidity formation as a measure of the amount of fibrinogen.
The present invention also provides a reagent for the simultaneous determination of fibrinogen and fibrinogen fission products, wherein it comprises 0.01 to 10 U snake venon enzyme with thrombin-like effectiveness per ml. of sample plasma, 0.5 to 4% by weight of a water-soluble polyalcohol, 1 to 20 mMole/liter of calcium ions and 0.05 to 0.3 mole/liter of buffer of pH 7.0 to 8.0, referred to the solution ready for use, as well as optionally a detergent.

REFERENCES:
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patent: 4289498 (1981-09-01), Baughman et al.
Wenzel et al, Deutsche Medizinsches Wochenschrift 99(1974), 746-56, (translation included).
Becker et al., Abstract 96(1):3311v.
Wenzel et al., Abstract only, Deutsche Medizinsches Wochenschrift, Apr. 12, 1974, 99(15), pp. 746-756.
Bergmeyer, Methods of Enzymatic Analysis, 3rd edition, vol. V, Verlag Chemie GmbH, D-6940 Weinheim, 1984, pp. 493-499.
Siefring et al., Clin Chem., 29/4, 614-617 (1983).
Exner et al., Am. J. Clin. Pathol., 71, 521-527 (1979).
Becker et al., Throm Res., 35; 475-484 (1984).
Marguerie et al., Biochim Biophy Acta., 579 (1979), 134-141.

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