Process and a reagent for the determination of low density lipop

Details Process and a reagent for the determination of low density lipop Process and a reagent for the determination of low density lipop

1984-10-02

1988-05-24

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 11, 435 28, 435 19, 435 26, 436 71, 436175, 436536, 436817, 436825, 436826, G01N 3392

Patent

active

047466057

ABSTRACT:
A method for determining low density lipoproteins (LDLs) in a body fluid sample, as well as a reagent suited for this use, are taught. The method involves precipitating high density lipoproteins (HDLs) from the sample, using an HDL specific antibody or reactive fragment, and then determining the presence and amount of LDL in the supernatant which results. The reagent contains the HDL specific antibodies, as well as polyanions and divalent cations.

REFERENCES:
patent: 4018662 (1977-04-01), Ruhenstroth-Bauer
patent: 4211530 (1980-07-01), Goverde
patent: 4399217 (1983-08-01), Holmquist
Mangold in Clinical Biochemistry Principals & Methods, vol. II, Walter de Gruyter, N.Y. 1974, pp. 1009-1017.
Kim, J. of Biological Chemistry 254(19), pp. 9621-9629, 1979.

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