Procedures for efficient separation of cells, cellular materials

Details Procedures for efficient separation of cells, cellular materials Procedures for efficient separation of cells, cellular materials

1996-07-05

1999-12-28

Naff, David M.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

210255, 210600, 210767, 435239, 435243, 4353081, 435814, 435261, 435372, B01D 2400, B01D 3700, C12N 502, C12N 508

Patent

active

06008040&

ABSTRACT:
A process and device are provided for achieving cascade flow to achieve rapid separations of target components from fluid mixtures is provided. The devices of the invention have a cascading flow channel. The separation processes of the invention may include singly or in combination, the step of adjusting the total voids volume in all elements employed in the devices of the invention to be equal to or less than 6% of the total volume of fluid that is to be processed. A single separation process or a combination of separation processes may be used where the fluid mixture is whole blood, whole blood that is diluted or is in some other way treated, concentrated, divided into two or more streams, or augmented with blood or blood components, cellular materials such as proteins, antibodies, enzymes and fractions thereof, and nuclear materials such as DNA, RNA and their fragments. These separation processes of the invention provide higher yields and purities of the desired products than has been possible by the prior art.

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