Procedure to preserve antigens of Entamoeba histolytica without

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 29, 435961, 435967, 436518, 436174, 436175, 436176, 530422, 530822, G01N 33569

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054590426

ABSTRACT:
The invention relates methods of determining Entamoeba histolytica antigenic reference patterns of proteins recognized by sera from patients having amebic liver abscesses, patients having intestinal amebiasis and patients negative for any amebic disease and methods using these antigenic reference patterns for aiding in the differential diagnosis of a patient with amebic liver abscesses or intestinal ambiasis by detecting the presence of antibodies in a sample of human serum which bind to Entamoeba histolytica proteins identified in the antigenic reference patterns.

REFERENCES:
Arguello-Garcia et al., "Evaluation of an immunoblot methodology for the detection of relevant Entamoeba histolytica antigens by antibodies induced in human amebiasis," Arch, Invest. Med., 21:3-9 (1990).
S. Said-Fernadez, et al., Zeitschrift Fur Parasitenkunde 56, 219-225 (1978).
H. M. Mathews, et al., J. Protozool., 33 (3), (1986), pp. 328-334.
Shiv Pillai, et al., Gastroenterology, 83, (1982), pp. 1210-1214.
Agneta Aust Kettis, et al., Am. J. Trop. Med. Hyg., 32 (3), (1983), pp. 512-521.
Eva E. Avila, et al., J. Protozool., 32 (1), (1985), pp. 163-166.

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