Procedure of separation of proteins by column chromatography usi

Details Procedure of separation of proteins by column chromatography usi Procedure of separation of proteins by column chromatography usi

1994-11-30

1997-09-23

Achutamurthy, Ponnathapura

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

Separation or purification

530416, 530417, C07K 114, C07K 116, C07K 118, C07K 134

Patent

active

056706316

ABSTRACT:
A chromatography column packing material and column chromatographic methods using a silica gel based column packing material are disclosed. The silica gel material is coated by a phospholipid bilayer containing at least two lipids, at least one having an electric charge. Proteins are separated using this column packing material without denaturing because of the lipid coating. The protein molecule binds to the lipid bilayer coated silica gel and is then eluted by raising the temperature.

REFERENCES:
patent: 5310648 (1994-05-01), Arniold et al.
Chemical Abstract; vol. 113, 147546T, 1990.

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