Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1995-04-20
1996-11-26
Zitomer, Stephanie W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 912, 536 243, 536 2433, C12Q 168, C12P 1934, C07H 2104
Patent
active
055784496
ABSTRACT:
A procedure is provided for the sex determination of embryos in mammals, that is particularly exemplified on bovines. The procedure overcomes the limitations given in former techniques. It includes a new stage of polymerase chain reaction PCR method, using specific oligonucleotides in order to amplify fragments corresponding to the sampled cells and not from false ones, spuriously produced. In this way, the necessary sensitivity is obtained. The oligonucleotides sequences are particularly selected for the procedure. The procedure is used for the sex determination of embryos in mammals, and the procedure is sensitive enough to be carried out a sample containing few cells, is highly secure, and which give positive results for both female and male sex; specially applied to bovine embryos.
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Fr asch Alberto C.
Ugalde Rodolfo A.
Hilding Ohlsson, S.A.
Rees Dianne
Walker Alfred M.
Zitomer Stephanie W.
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