Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Patent
1998-03-05
2000-08-15
Fox, David T.
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
46 6, 800278, 800298, 800316, 435430, 435431, 435469, C12N 1582, C12N 1584, A01G 106, A01H 500
Patent
active
061039551
ABSTRACT:
The procedure is based on the use, as vegetable starting material for the transformation, of the first shoots from the graft of buds of adult trees onto stock, the genetic transformation of explants from the adult shoots by mean of co cultivation with Agrobacterium tumefaciens in mother plaques, and the obtaining of complete adult woody plants by means of in vitro micrografting of the transgenic buds, apices or shoots, regenerated by means of the explants, by means of in vitro micrograft onto stock cultivated in vitro.
This procedure makes it possible to avoid the juvenile period and the high heterozygosis which affects most woody species, blossoming and fruiting of the transgenic plants are brought forward, and it permits the direct genetic transformation of commercially interesting varieties.
It has argicultural applications.
REFERENCES:
Cervera et al, Transgenic Research, vol. 7, pp. 51-59, 1998.
Cervera et al, Physiol. Mol. Plant Pathol., vol. 52, pp. 67-78, 1998.
Landrige et al, Plant J., vol. 2, pp. 631-638, 1992.
Mahlstede et al, Graftage, In: Plant Propagation, (J.P. Mahlstede and E. S. Haber, eds.), John Whily & Sons: New York, pp. 239-274, 1957.
Newell et al, Plant Cell Rep., vol. 10, pp. 30-34, 1991.
Hood et al, Bacteriol., vol.168, pp. 1291-1301, 1986.
Potrykus, I., Ann. Rev. Plant Physiol. Mol. Biol., vol. 42, pp. 205-225, 1991.
Pena et al, Plant Cell Rep., vol. 14, pp. 616-619, 1995.
Cervera Ocana Magdalena
Duran Vila Nuria
Juarez Roldan Jose
Navarro Lucas Antonio
Navarro Lucas Luis
Fox David T.
Instituto Nacional De Investigacion Y Technologia Agraria Y Alim
Instituto Valencia De Investigaciones Agrarias (IVIA)
Mehta Ashwin
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