Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2011-08-09
2011-08-09
Chunduru, Prabha (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S022100
Reexamination Certificate
active
07993827
ABSTRACT:
The present invention describes a method for the determination of DNA fragmentation in animal sperm. It particularly refers to a procedure to evaluate the integrity of the chromatin/DNA of the sperm by means of a treatment of the sample with a denaturing solution followed, optionally by a stain; a subsequent treatment with a lysis solution that does not contain a protein denaturing detergent, followed, optionally, by a stain; and an evaluation of the integrity of the chromatin/DNA. The present invention also refers to a Kit to evaluate the quality of the sperm of animals which includes a DNA denaturing solution and a lysis solution that does not contain a protein denaturing detergent.
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Fernandez, J.L. et al. The sperm chromatin dispersion test: a simple method for the determination of sperm DNA fragmentstion. J. Andrology, vol. 24, No. 1, pp. 59-66, 2003.
Bezanehtak, H. et al. Study of demembranated, reactivated human spermatozoa with decondensed nuclei. J. Exp. Zool., vol. 284(7), pp. 789-797, 1999.
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Fernandez Garcia Jose Luis
Gosalvez Berenguer Jaime
Goyanes Villaescusa Vicente
Chunduru Prabha
Ladas & Parry LLP
Universidad Autonoma de Madrid
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