Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-02-14
1998-02-03
Campbell, Eggerton A.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 912, C12Q 170, C12Q 168, C12P 1934
Patent
active
057143129
ABSTRACT:
A method for detecting and quantifying viral or subviral pathogens in the test sample in which antibodies against a component of the viral or subviral pathogen is immobilized on a substrate. The test sample is placed in contact with the immobilized antibody and then a DNA primer complementary to a fragment or the total genome of the viral or subviral pathogen is permitted to hybridize to the RNA or DNA of the immobilized viral or subviral pathogen. The DNA is then amplified and the amplification products are detected and quantified.
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Ann. Appl. Biol., vol. 117, 1990, Vunsh, R. et al.: "The Use of Polymerase Chain Reaction (PCR) For The Detection Of Bean Yellow Mosaic Virus In Gladiolus", pp. 561-569.
Proc. Natl. Acad. Sci. USA, vol 90, pp. 10168-10172, Nov. 1993, Agricultural Sciences, Passmore, et al., "Beet western yellows virus-associated RNA . . . ".
Current Communications in Molecilar Biology, Cold Spring Harbor Laboratory, 1983, "Plant Infectious Agents: Viruses, Viroids, Virusoids, and Satellites."
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Borja Tome Maria Jose
De Blas Beorlegui Carmen
Nuno Bardosa Nolasco Gustavo
Pons Ascaso Fernando
Torres Pascual Vincente
Campbell Eggerton A.
Instituto Nacional de Investigacion y Techologia Agraria y Alime
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