Procedure for preparing gene expression libraries enriched for s

Details Procedure for preparing gene expression libraries enriched for s Procedure for preparing gene expression libraries enriched for s

1997-07-25

2000-03-21

McKelvey, Terry

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4353201, C12Q 168, C12N 1563

Patent

active

060401443

ABSTRACT:
The present invention relates to a method for producing a gene expression library enriched for a particular class of genes comprising (i) a nucleic acid preparation step and (ii) a polyclonal antiserum screening step, wherein both steps define the class of genes of interest. The method of the invention, using selection procedures directed not only at the underlying nucleic acid but also a functional attribute of the target class of proteins, may be used to produce gene expression libraries enriched for genes encoding a desired class of proteins, including, but not limited to, secretory proteins, cell cycle associated proteins, proteins defined by a particular subcellular location, tissue specific proteins, infection-related proteins, and non-infectious disease associated proteins.

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