Procedure for normalization of cDNA libraries

Details Procedure for normalization of cDNA libraries Procedure for normalization of cDNA libraries

1995-06-06

1997-12-30

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 9151, 4351723, 536 231, 536 235, C12Q 168, C12P 1934, C12N 1500, C07H 2100

Patent

active

057028982

ABSTRACT:
This invention provides a method to normalize a cDNA library constructed in a vector capable of being converted to single-stranded circles and capable of producing complementary nucleic acid molecules to the single-stranded circles comprising: (a) converting the cDNA library in single-stranded circles; (b) generating complementary nucleic acid molecules to the single-stranded circles; (c) hybridizing the single-stranded circles converted in step (a) with complementary nucleic acid molecules of step (b) to produce partial duplexes to an appropriate Cot; (e) separating the unhybridized single-stranded circles from the hybridized single-stranded circles, thereby generating a normalized cDNA library.

REFERENCES:
patent: 5482845 (1996-01-01), Soares et al.
Patanjeli et al. Proc. Natl. Acad. Sci. USA 88 1943-1947 (1991) Construction of a uniform abundance (normalized) cDNA library.
Haraguchi et al. Proc. Natl. Acad. Sci. USA 84 412-415 (1987) Molecular cloning and nucleotide Sequence of cDNA for human . . .

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