Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing a carotene nucleus
Patent
1998-07-30
2000-02-08
Lilling, Herbert J.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing a carotene nucleus
4352571, C12P 2300
Patent
active
06022701&
DESCRIPTION:
BRIEF SUMMARY
FIELD OF THE INVENTION
The present invention concerns a process for cultivating Haematococ cells for the large-scale production of astaxanthin. The present invention also relates to a process for the large-scale production of astaxanthin-enriched Haematococcus cells.
BACKGROUND OF THE INVENTION AND PRIOR ART
Haematococcus is a group of green microalgae which are able to accumulate a large amount of a keto-carotenoid, astaxanthin, under certain environmental conditions (Boussiba et aL 1992). Astaxanthin is the major pigment imparting the red-pinkish color of salmon, trout and shrimp, whose prices are largely determined by their color. Currently, the major commercially available source of astaxanthin is in the form of a synthetic product, which is expensive (>US$ 3,000 per kilogram) and may contain astaxanthin compounds having an unnatural configuration. There is now a trend toward using natural sources of feed nutrients for the purpose of preparing feed for the above types of fish, and thus a less expensive and natural source of astaxanthin has been sought. This group of green microalgae appears to be the most promising source for this purpose. Although astaxanthin can be synthesized by other algae, bacteria and a few fungi (Schroeder & Johnson 1993; Yokoyama & Miki 1995), the amounts accumulated by Haematococcus (Boussiba & Vonshak 1991), however, surpass those obtained in the above noted sources. According to the present development of aquaculture, it is expected that by the year 2000, more than 100 tons of astaxanthin will be required (Johnson & An, 1991; Borowitzka, 1992). This potential demand could open a large market for microbially produced astaxanthin. Several reasearch groups have attempted to establish large production systems for Haematococcus.
However, no one has succeeded so far, due to the difficulties in enhancing astaxathin accumulation rate, preventing contamin ation by other microorganisms, and failure in special photobioreator design. An example of previous methods for producing astaxanthin and other pigments from Haematococcus is that described in the international patent application No. WO 89/06910.
H. pluvialis has a unique life cycle comprising two stages: a green, motile, vegetative stage in which the cells continuously divide and synthesize chlorophyll and a red, non-motile resting stage (cyst) in which cell division stops, chlorophyll content remains constant and astaxanthin content and cellular dry weight continuously increase. These two stages are illustrated in FIG. 1, which depicts graphically the relationship between four parameters of the growth of H. pluvialis, namely, chlorophyll, astaxanthin, dry weight and cell number. The optimal environ mental and nutritional conditions required for these two stages are quite different. For vegetative growth, full nutrient medium, moderate light intensity and adequate temperature and pH are essential. For the resting stage, however, no nutrient (except carbon) is required and higher light intensity (e.g., sunlight) is necessary for faster astaxanthin accumulation. Due to such discrepancies, the two stages must be separated into different cultivating systems with different medium accordingly. The growth strategies for each stage are also different. In the green stage, the optimal conditions for cell division are maintained to achieve the ma um cell number. In the red stage, the optimal inductive conditions must be provided for astaxathin accumulation. Heretofore, no such process has been developed for the effective large scale, two-stage or two-phase growth of Haematococcus cells, which answers the demands of each of the above growth stages of Haematococcus cells. The known processes suffer from a number of drawbacks, including the need for expensive growth media, apparatuses, use of sterile equipment and media and/or the use of expensive antibiotics or other anti-microbial agents to prevent contamination of the desired Haematococcus cultures by other microorganisms such as other microalgae, fungi, yeasts and bacteria. Thus, such
REFERENCES:
"Algal biotechnology products and processes--matching science and economi Borowitzka, et al. Journal of Applied Phycology 4: 267-279, 1992.
"Critical Reviews in Biotechnology" Johnson, et al. Critical Reviews in Biotechnology, 11(297-326) (1991).
"Astaxanthin Production by a Green Alga, Haematococcus plubialis Accompanied with Morphological Changes in Acetate Media" Kobayashi, et al. Journal of Fermentation and Bioengineering, vol. 71, No.5, 335-339 (1991).
"A new tubular reactor for mass production of microalgae outdoors" Richmond, et al. Journal of Applied Phycology 5: 327-332, 1993.
"Antioxidant role of cartenoids in Phaffia rhodozyma" Schroeder, et al. Journal of General Microbiology 139, 907-912, 1993.
"A Vertical Alveolar Panel (VAP) for Outdoor Mass Cultivation of Microalgae and Cyanobacteria" Tredici, et al. Bioresource Technology 38, 153-159 (1991).
"Composition and presumed biosynthetic pathway of carotenoids in the astaxanthin--producing bacterium Agrobacterium aurantiacum" Yokohama, et al. FEMS Microbiology Letters 128, 139-144 (1995).
Derwent Computer Biotechds Abstract 92-03349 Boussiba et al "Astaxanthin accumulation in the green alga Haematococcus pluvialis; induction of pigment production" Plant Cell Physiol. (1991) 32, 7, 1077-82.
Derwent Computer Biotechds Abstract 94-13293 borowitzka "Large-scale algal culture systems; the next generation; alga e.g. Phaeodactylum tricornutum large-scale culture in a tubular photoreactor fermentor" Australas. Biotechnol.; (1994) 4, 4, pp. 212-215.
Derwent Computer Biotechds Abstract 89-06550 WO 8901977 (Mar. 9, 1989).
"Effect of temperature and irradiance on growth of Haematococcus pluvialis (Chlorophyceae)." Journal of Phycology, 30 (5). 1994 pp. 829-833 (Abstract) Biosis 95: 26587.
"Astaxanthin prepn. for medical antioxidant--by aerobic culture of Haematococcus pluvialis in the dark, then in light with carbon and active oxygen source to induce cyst formation." Database WPI, Section Ch, Week 9317. Derwent Publications Ltd. (Abstract)J05068585, Mar. 23, 1993.
"Enhancement and Determination of Astaxanthin Accumulation in Green Alga Haematococcus pluvialis. " Sammy Boussiba, et al. Methods in Enzymology. vol. 213. Carotenoids. pp. 386-391, 1992.
"Autotrophic growth and carotenoid prodution of Haematococcus pluvialis in a 30 liter air-lift photobioreactor." Mark Harker, et al. Chemical Abstracts, vol. 125, No. 17. XP002031369. (Oct. 21, 1996) CA(125):219715v.
"Astaxanthin accumulation in the green alga Haematococcus pluvialis." Sammy Boussiba, et al. Chemical Abstracts, vol. 115, No. 25. XP002031370. (Dec. 23, 1991)CA115: 275484z.
"Astaxanthin prodn.--comprises culturing green algae by temp. stress and induction of cyst formation in presence of active oxygen source and carbon source." Database WPI. Section Ch, Week 9516. Derwent Publications Ltd. (Abstract) J07039389(Feb. 10, 1995).
Boussiba Sammy
Cohen Zvi
Richmond Amos
Vonshak Avigad
Ben-Gurion University of the Negev Research and Development Auth
Lilling Herbert J.
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