Procedure for controlling the enumeration of the absolute...

Details Procedure for controlling the enumeration of the absolute... Procedure for controlling the enumeration of the absolute...

2005-07-05

2005-07-05

Campell, Bruce R. (Department: 1654)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

C435S049000

Reexamination Certificate

active

06913901

ABSTRACT:
Procedure for controlling the enumeration of the absolute count of cells (or other particles) present in a sample. The procedure consists of the following: to prepare, in known quantities and proportions, a mixture or stock solution of two or more populations of reference particles of differing characteristics; to add a known quantity of this mixture of reference particles to a known volume of the sample which contains the cells (or other particles) to be counted; to measure, in a flow cytometer, the sample containing both the cells (or other particles), the events of which are to be counted, and the mixture of different populations of reference particles; to calculate the absolute number of cells (or other particles) present in the sample to be counted and; to check that the proportion between the different reference particles present in the sample measured concurs with the proportion that exists between them in the initial mixture or stock solution which contained the different populations of reference particles prior to adding it to the sample.

REFERENCES:
patent: 5882863 (1999-03-01), Imai et al.
patent: 0586183 (1994-03-01), None
patent: 0586183 (1994-09-01), None
patent: 2795820 (2001-01-01), None
European Search Report EP 02 38 0032 dated Apr. 23, 2002.
“Cytofluorometric Methods for Assessing Absolute Numbers of Cell Subsets In Blood”, Bruno Brando et al., pp. 327-345.

Affiliated with

Also associated with

Rating

Procedure for controlling the enumeration of the absolute... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Procedure for controlling the enumeration of the absolute..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Procedure for controlling the enumeration of the absolute... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3374576