Procedure for automated solid-phase immunoassay using a centrifu

Chemistry: analytical and immunological testing – Automated chemical analysis – Utilizing a centrifuge or compartmented rotor

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422 69, 422 71, 422 72, 494 37, G01N 3500, G01N 3096

Patent

active

052583090

ABSTRACT:
A method for conducting immunoassays in an automated fashion is disclosed. A biological fluid under test is placed in a tube (11) which has a solid support (18) therein to which a specific analyte in the biological fluid will be selectively bound. After the analyte in the biological fluid is bound to the solid support (18), the tube (11) is rotated at high speed about its longitudinal axis causing the biological fluid to be transported up the inside walls of tube (11), over the open terminal end (16), and into waste chamber (15). The solid support (18) with the bound analyte may easily be washed by flushing the inside of tube (11) with water or other suitable fluids and rotating the tube (11) at high speed about its longitudinal axis.

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