Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...
Reexamination Certificate
2006-04-04
2009-10-06
Cook, Lisa V (Department: 1641)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving antigen-antibody binding, specific binding protein...
C435S007210, C435S283100, C436S501000, C436S518000, C422S050000, C422S067000
Reexamination Certificate
active
07598044
ABSTRACT:
In a procedure for determining the concentrations of ligands contained in a sample wherein a first ligand is bond-specific to a first receptor and a second ligand is bond-specific to a second receptor, the first receptor is immobilized on a first test site and the second receptor is immobilized on a second test site on a substrate. A competitor that is bond-specific to the second receptor is mixed with the sample so that it is present in a predetermined concentration in the mixture. The mixture is brought into contact with the substrate so that the first ligand and the second ligand and/or the competitor bind to the first receptor and the second receptor, respectively. Afterwards the unbound components of the mixture are removed from the substrate. A first metering signal relative to the concentration of the first ligand bound to the first receptor and a second metering signal relative to the concentration of the competitor bound to the second receptor are then generated. The concentrations of the ligands in the sample are determined with reference to the metering signals and the known concentration of the competitor in the mixture.
REFERENCES:
patent: 6197503 (2001-03-01), Vo-Dinh et al.
patent: WO 92/18866 (1992-10-01), None
patent: WO 95/18377 (1995-07-01), None
patent: WO 95/22766 (1995-08-01), None
patent: WO 95/22766 (1995-08-01), None
patent: WO 2004/004455 (2004-01-01), None
Cook Lisa V
Micronas Holding GmbH
The Webb Law Firm
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