Procaryotic leader sequence in recombinant baculovirus expressio

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 697, 4352351, 4352402, 4353201, 536 221, 536 231, 536 241, C12P 2106, C12N 700, C12N 500, C12N 1500

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053227743

ABSTRACT:
A process for the preparation of a protein, which process comprises culturing under such conditions that the protein is obtained cells infected with a recombinant baculovirus having a promoter, capable of directing the expression of a gene in the cells, operably linked to a heterologous gene which encodes the protein and which is preceded by a leader sequence comprising: T A A T C A T C C A C A G G A G A C T T T C T G [SEQ. ID NO: 1].

REFERENCES:
patent: 4879236 (1989-11-01), Smith et al.
Kozak "Compilation and analysis . . . " NAR 12:2 1984 pp. 857-872.

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