Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Bacterium or component thereof or substance produced by said...
Reexamination Certificate
2011-05-31
2011-05-31
Graser, Jennifer E (Department: 1645)
Drug, bio-affecting and body treating compositions
Antigen, epitope, or other immunospecific immunoeffector
Bacterium or component thereof or substance produced by said...
C424S093100, C435S243000, C435S252100
Reexamination Certificate
active
07951385
ABSTRACT:
The invention relates to a strain ofEnterococcus mundtiihaving probiotic qualities. The strain ofE. mundtii(ST4SA) produces an antimicrobial peptide which exhibits antimicrobial activity against a broad range of bacteria. The invention also provides an isolated nucleotide sequence which codes for the antimicrobial peptide (peptide ST4SA). Another aspect of the invention relates to a process for the production of a peptide of the invention which comprises cultivatingEnterococcus mundtiistrain ST4SA in a nutrient medium under micro-aerophilic conditions at a temperature of between 100 C. and 45° C., until a recoverable quantity of said peptide is produced, and recovering said peptide. The isolated peptide of the invention may be used as an antimicrobial agent in a liquid formulation or a gel formulation as a topical treatment and may also be used as an antimicrobial agent following encapsulation in a polymer.
REFERENCES:
patent: 6878373 (2005-04-01), Keeton et al.
patent: 6919080 (2005-07-01), Tokunaga et al.
patent: 6919427 (2005-07-01), Mao et al.
patent: 6929793 (2005-08-01), Spivey-Krobath et al.
patent: 6960341 (2005-11-01), Viscomi et al.
patent: 2003 339377 (2003-12-01), None
patent: WO 03/035681 (2003-05-01), None
Kawato Shinichi et al:“Biochemical and genetic characterization of mundticin KS, an antilisterial peptide produced byEnterococcus mundtiiNFRI 7393”, Aug. 2002, vol. 68 No. 8, pp. 3830-3840, Applied and Environmental Microbiology.
Saavedra Lucila et al: “Enhancement of the enterocin CRL35 activity by a synthetic peptide derived from the NH2-terminal sequence” Jul. 2004, vol. 48, No. 7, pp. 2778-2781, Antimicrobial Agents and Chemotherapy.
Todorov S D et al: “An antibacterial and antiviral peptide produced byEnterococcus mundtiiST4V isolated from soya beans” Jun. 2005, vol. 25, No. 6 pp. 508-513, International Journal of Antimicrobial Agents, Amsterdam, NL.
Zendo T et al: “Identification and production of a bacteriocin fromEnterococcus mundtiiQU 2 isolated from soybean” 2005, vol. 99, No. 5, pp. 1181-1190, Journal of Applied Microbiology.
De Kwaadsteniet et al: “Characterization of a 3944 Da bacteriocin, produced byEnterococcus mundtiiST15, with activity against Gram-positive and Gram-negative bacteria” Dec. 15, 2005, vol. 105, No. 3, International Journal of Food Microbiology, Elsevier Science Publishers, Amsterdam, NL.
Van Reenen, et al. “Isolation, purification and partial characterization of plantaricin 423, a bacteriocin produced byLactobacillus plantarum.” 1998, pp. 1131-1137, 84,J. Appl. Microbiol.
Verellen, et al “Fermentation optimization of plantaricin 423, a bacteriocin produced byLactobacillus plantarum423” 1998, pp. 174-179, 86,J. Ferment. Bioeng.
Nel, et al, “Effect of bacteriocins pediocin PD-1, plantaricin 423, and Nisin on biofilms ofOenococcus oenion a stainless steel surface.” 2002, pp. 191-196, 53,Am. J. Enol. Viticult.
Van Reenen, et al,. “Characterization and heterologous expression of a class IIa bacteriocin, plantaricin 423 fromLactobacillus plantarum423, inSaccharomyces cerevisiae.” 2002, 29-40, 81,Int. J. Food Microbiol.
Brink, M., S.D. Todorov, M. Senekal and L.M.T. Dicks. 2006. The effect of prebiotics on the production of antimicrobial compounds, resistance to growth at low pH and in the presence of bile, and adhesion of probiotic cells to intestinal mucus.J. Appl. Microbiol. 100: 813-820.
Mare, L., G.M. Wolfaardt, L.M.T. Dicks. 2006. Adhesion ofLactobacillus plantarum423 andLactobacillus salivarius241 to the intestinal tract of piglets, as recorded with fluorescent in situ hybridization (FISH), and production of plantaricin 423 by cells colonized to the ileum.J. Appl. Microbiol. 100: 838-845.
Powell, J., Todorov, S.D., Van Reenen, C.A., Dicks, L.M.T., Witthuhn, C. 2006. Growth inhibition ofEnterococcus mundtiiin kefir by in situ production of bacteriocin ST8KF.Lait. 86: 401-405.
Van Reenen, C.A., Van Zyl, W.H., Dicks L.M.T. 2006. Expression of the immunity protein of plantaricin 423, produced byLactobacillus plantarum423, and analysis of the plasmid encoding the bacteriocin.Applied and Environmental Microbiology. 72: 7644-7651.
Van Reenen, C.A. and Dicks, L.M.T 1996 Evaluation of numerical analysis of random amplified polymorphic DNA (RAPD)-PCT as a method to differentiateLactobacillus plantarumandlactobacillus pentosus. Curr Mictrobiol. 32:183-187.
Dicks, LMT., F.D. Mellett and L.C. Hoffman 2003 Use of bacteriocin-producing starter cultures ofLactobacillus plantarumandLactobacillus curvatusin production of ostrich meat salami.Meat Science66:703-708.
Bauer, et al “Control of malolactic fermentation in wine.” 2004, pp. 74-88, 25,S.A. J Enol. Vitic..
Bauer, et al,. “Mode of action of lipid II-targeting lantibiotics.”, 2005, pp. 201-216, 101,Int. J. Food Microbiol.
M. Granger, S.D. Todorov, M.K.A. Mathews, L.M.T. Dicks. 2005. Growth ofEnterococcus mundtiiin medium filtrate and purification of bacteriocin ST15 by cation exchange chromatography.Journal of Basic Microbiol. 45: 419-425.
Brink, M., S.D. Todorov, M. Senekal and L.M.T. Dicks. 2006. The effect of prebiotics on the production of antimicrobial compounds, resistance to growth at low pH and in the presence of bile, and adhesion of probiotic cells to intestinal mucus.J. Appl. Microbiol. 100: 813-820.
Todorov, S.D and L.M.T. Dicks. 2006 Medium components effecting bacteriocin production by two strains ofLactobacillus plantarumST414BZ and ST664BZ isolated from bozaBiologia61:3:269-274.
Powell, J., Witthuhn, C., Todorov, S.D., Dicks, L.M.T., 2006. Characterization of bacteriocin ST8KF produced by a kefir isolateLactobacillus plantarumST8KF.Int Dairy Journal17: 190-198.
Brink Marelize
Dicks Leon Milner Theodore
Knoetze Hendriette
Todorov Svetoslav Dimitrov
Cipla Medpro Research and Development (Pty) Ltd.
Graser Jennifer E
Schulman B. Aaron
Stites & Harbison PLLC
Weyer Stephen J.
LandOfFree
Probiotic strain and antimicrobial peptide derived therefrom does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Probiotic strain and antimicrobial peptide derived therefrom, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Probiotic strain and antimicrobial peptide derived therefrom will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2630680