Probes labeled with energy transfer coupled dyes

Details Probes labeled with energy transfer coupled dyes Probes labeled with energy transfer coupled dyes

1996-05-08

2000-02-22

Campbell, Eggerton A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 6, C07H 2102, C12Q 168

Patent

active

060281901

ABSTRACT:
Fluorescent labels having at least one donor and at least one acceptor fluorophore bonded to a polymeric backbone in energy transfer relationship, as well as methods for their use, are provided. Of particular interest are the subject labels wherein the polymeric backbone is a nucleic acid and the donor fluorophore is bonded to the 5' terminus of said nucleic acid. Such labels find use as primers in applications involving nucleic acid chain extension, such as sequencing, PCR and the like.

REFERENCES:
patent: 4996143 (1991-02-01), Heller et al.
patent: 5326692 (1994-07-01), Brinkley et al.
patent: 5401847 (1995-03-01), Glazer et al.
patent: 5538848 (1996-07-01), Livak et al.
patent: 5573909 (1996-11-01), Singer et al.
patent: 5591578 (1997-01-01), Meade et al.
patent: 5614386 (1997-03-01), Metzker et al.
Stryer (1978) Ann. Rev. Biochem. 47:819-46.
Ozaki et al. (1992) Nucleic Acids Research 20:5205-14.
Ju et al. (1995) Proc. Natl. Acad. Sci. USA 92:4347-51.
Geoghegan et al. (1993) Bioconjugate Chemistry 4:537-44.
Murakami et al. (1994) Nucl. Acids Symp. Series No. 31, pp. 55-56.
Wang et al. (1996) Electrophoresis 17:1485-90.
Metzker et al. (Mar. 1996) Science 271:1420-22.
Hochstrasser (1994) Proc. SPIE--Int. Soc. Opt. Eng., 2137:775-81.
Maliwal et al. (1993) Biophysical Chemistry 46:273-81.
Parkhurst et al. (1995) Biochemistry 34:293-300.
Wu et al. (1996) J. Biol. Chem. 271:1470-74.
Bagwell et al. (1994) Nucleic Acids Research 22:2424-5.
Matsuoka et al. (1994) Tetrahedron: Asymmetry 5:2335-8.
Lee et al. (1994) Analytical Biochemistry 220:377-383.
Rye et al, Nucleic Acids Res 19: 327-333, 1990.

Affiliated with

Also associated with

Rating

Probes labeled with energy transfer coupled dyes does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Probes labeled with energy transfer coupled dyes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Probes labeled with energy transfer coupled dyes will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-521700