Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1993-12-01
1998-12-22
Houtteman, Scott W.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
536 237, C12Q 168
Patent
active
058517618
ABSTRACT:
The invention provides nucleotide probes, kits and methods for the detection and differentiation of Mycobacteria. The gene probes, kits and methods are useful for the diagnosis of tuberculosis and/or for epidemiological study tools for investigating the progress of infections caused by Mycobacteria.
The gene probes as provided comprise part or all of nucleotide sequences provided in the soecification or an allele or a derivative of the nucleotide sequences.
The gene probes can distinguish between M.tuberculosis, M.bovis and BCG as well as being able to distinguish between different strains of M.tuberculosis. The probes do not show significant hybridisation to nucleic acids from M.paratuberculosis, M.intracellulare, M.scrofulaceum, M.phlei, M.fortuitum, M.kansasii, M.avium, M.malnioense. M.flavescens, M.gordonae and M.chelonei.
REFERENCES:
Journal of General Microbiology, vol. 135, No. 9, Sep. 1989, SGM (GB), Z.F. Zainuddin et al.: "Polymorphic repetitive DNA sequences in Mycobacterium tuberculosis detected with a gene probe from a Mycobacterium fortuitum plasmid".
Journal of Clinical Microbiology, vol. 265, No. 11, Nov. 1988, American Society for Microbiology, K.D. Eisenach et al.: "Repetitive DNA sequences as probes for Mycrobacterium tuberculosis".
Nucleic Acids Research, vol. 18, No. 1, Jan. 1990, Oxford Press, (GB) D. Thierry et al.: "IS6110, an IS-like element of Mycobacterium tuberculosis complex".
Biological Abstracts, vol. 87, 1989, (Philadelphia, PA, US), R. P. Prabhakara et al.: "Repetitive DNA sequences of Mycrobacterium tuberculosis: Analysis of differential hybridization patterns with other mycrobacteria", Abstract 103147.
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Kent et al., "Demonstration of Homology between IS6110 of Mycobacterium tuberculosis and DNAs of Other Mycobacterium spp.," Journal of Clinical Microbiology, 33(9):2290-2293 (1995).
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Torrea et al., "Chromosomal DNA Fingerprinting Analysis Using the Insertion Sequence IS6110 and the Repetitive Element DR as Strain-Specific Markers for Epidemiological Study of Tuberculosis in French Polynesia," Journal of Clinical Microbiology, 33(7):1899-1904 (1995).
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Catty David
Dale Jeremy Watson
McAdam Ruth Anne
Zainuddin Zainul Fadziruddin Bin
Brunelle Jan P.
Cogent Limited
Dreger Walter H.
Houtteman Scott W.
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