Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-08-13
1998-09-29
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 911, 435 6, 435 5, 536 231, 536 243, 536 2433, 536 2432, C12P 1934, C12Q 168, C07H 2104, C07H 2102
Patent
active
058144927
ABSTRACT:
A method for reducing background caused by target-independent generation of amplification products, typically the products of a ligase chain reaction or a polymerase chain reaction, involves chemically "masking" or blocking the amplification probes or primers so that they cannot be extended or ligated until the occurrence of a triggering event which can be delayed until the amplification reaction is begun. The probe masks take the form of complementary blocking oligonucleotides that are incapable of serving as template themselves and inhibit random tailing of the probe/primers. The blocking oligo masks are denatured from the probes during amplification and preferably are effectively eliminated from competing for probes in the amplification reaction.
REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 5035996 (1991-07-01), Hartley
patent: 5118801 (1992-06-01), Lizardi et al.
patent: 5142047 (1992-08-01), Summerton et al.
patent: 5166315 (1992-11-01), Summerton et al.
patent: 5185444 (1993-02-01), Summerton et al.
patent: 5215899 (1993-06-01), Dattagupta
patent: 5217866 (1993-06-01), Summerton et al.
patent: 5235033 (1993-08-01), Summerton et al.
patent: 5338671 (1994-08-01), Scalice et al.
patent: 5340728 (1994-08-01), Grosz et al.
patent: 5348853 (1994-09-01), Wang et al.
patent: 5418149 (1995-05-01), Gelfand et al.
patent: 5427929 (1995-06-01), Richards et al.
patent: 5427930 (1995-06-01), Birkenmeyer et al.
Clark, J.M., "Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases", Nucleic Acids Research, 16(20):9677-9686 (1988).
Abravaya et al. (1995) Nucl. Acids Res. 23:675-82.
Don et al. (1991) Nucl. Acids Res. 19:4008.
Chou, Q., et al. "Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications", Nucleic Acids Research, 20(7):117-1723 (1992).
Fahy, E., "Self-sustained Sequence Replication (3SR): An Isothermal Transcription-based Amplification System Alternative to PCR", PCR Methods and Applications, 1:25-33 (1991).
Kievits, T., "NASBA.TM. isothermal enzymatic in vitro nucleic acid amplification optimized for the diagnosis of HIV-1 infection", Journ. of Virol. Methods, 35:273-286 (1991).
Lizardi, P. M., "Exponential Amplification of Recombinant-RNA Hybridization Probes", Bio/Technology, 6:1197-1202 (1988).
Walker, G. T., "Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system", Proc. Natl. Acad. Sci. USA, 89:392-396 (1992).
Brainard Thomas D.
Carrino John J.
Abbott Laboratories
Brainard Thomas D.
Jones W. Gary
Rees Dianne
Yasger Paul D.
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