Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Calorimeter
Patent
1989-01-03
1992-07-07
Housel, James C.
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Calorimeter
422 52, 422 8206, 422 8207, 422 82, 422 08, 422 86, 2504591, 2504611, 356317, G01N 2164, G01N 3352
Patent
active
051281023
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to a probe for measuring the oxygen concentration in a solution or a gas by utilizing quenching by oxygen of luminescence following excitation by light.
2. Description of the Prior Art
Heretofore, as for a method for measuring the oxygen concentration in an aqueous solution, there has generally and widely been used an electrochemical method in which an oxygen electrode represented by Clark type one is used and reduction current of oxygen is measured under a controlled electric potential. However, in this method, since an electric current which flows between two electrodes is measured, the current density cannot be so diminished, and therefore miniaturization of the electrodes to be used is limited. Further, an electric current is generated even in a small extent, and thus it is not proper from the safety aspect to use the method, for example in vivo or in a blood vessel.
In view of these problems, various methods utilizing light for measuring oxygen concentration have been proposed. Since it was reported that pyrenebutyric acid is effective as a fluorescent probe for measuring oxygen concentration (W. M. Vaugham and G. Weber, Biochem, 9, 464 (1970)), many researches have been conducted on probes utilizing quenching by oxygen of fluorescence from derivatives of pyrene. Further, another device utilizing pyrenebutyric acid was made (N. Opitz and D. W. Lubbers, Z. Biomed. Techn. 28 (31), (1983)). However, in this method it is necessary to use an ultraviolet ray as the exciting ray, so that materials which transmit an ultraviolet ray must be used in the optical system such as a conductor of the exciting ray. When a probe comprising perylene dibutyrate adsobed on silica gel is used, a visible ray (wave length of 468 nm) can be used as the exciting ray (J. I. Peterson, R. V. Fitzgerald and D. K. Buckhold, Anal. Chem. 56, 62 (1984)). However, since this probe greatly suffers from water, it is necessary to protect it with a hydrophobic and oxygen-permeable membrane, which makes the miniaturization thereof more difficult. Thus, there has not hithereto been a probe for measuring oxygen concentration where the exciting ray and luminescence are visible rays and which can stably be used even when it is directly immersed in a liquid to be examined without a protective membrane such as an oxygen-permeable membrane thereon.
SUMMARY OF THE INVENTION
Accordingly, an object of the present invention is to provide a probe for measuring oxygen concentration, which can be used in a method for measuring oxygen concentration using light but not using an electrochemical method, where the exciting ray and luminescence are visible rays, so that a flexible plastic optical fiber and the like can be used as the conduction of light and also inexpensive materials for visible rays can be used in the optical system, and which can stably be used even when it is directly immersed in a solution and the like without a protective membrane such as an oxygen-permeable membrane, which makes the miniaturization thereof easier.
It has been found that the above object of the present invention is attained by a probe for measuring oxygen concentration comprising an immobilized polypyridine metal complex.
BRIEF DESCRIPTION OF THE DRAWINGS
FIG. 1 is a drawing illustrating an instrument for measuring the luminescence intensity of a probe for measurement of oxygen concentration. In FIG. 1, 1 represents a quartz plate, 2 represents a fixed membrane of a polypyridine metal complex, 3 represents a quartz cell, and 4 represents a holder made of rubber.
FIGS. 2 and 3 are drawings illustrating the relation between the oxygen concentration and the luminescence intensity in a solution to be examined using a probe for measurement of oxygen concentration of the present invention.
FIG. 4 is a drawing illustrating the relation between th oxygen concentration and the illuminescence intensity in a gas to be examined using a probe for measurement of oxygen concentration of the presen
REFERENCES:
patent: 4721669 (1988-01-01), Barton
patent: 4772707 (1988-09-01), Hammon et al.
patent: 4880752 (1989-11-01), Keck et al.
Russian Chemical Reviews, vol. 41, No. 11, Nov., 1972, pp. 991-1008, London, GB; A. T. Philipenko et al., "Analytical Chemistry of Metal Complexes with Nitrogen-containing Ligands of the 2,2' bipyridyl type".
Kaneko Masao
Nakamura Hideki
Housel James C.
Redding David
Rikagaku Kenkyusho
Terumo Kabushiki Kaisha
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