Probe for DNA and a process for the detection of "shigellae" and

Details Probe for DNA and a process for the detection of "shigellae" and Probe for DNA and a process for the detection of "shigellae" and

1985-07-12

1989-03-28

Warden, Robert J.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 29, 4351723, 435810, 435320, 436501, 436808, 536 27, 935 29, 935 78, C12Q 168, G01N 3353, C07H 1512

Patent

active

048163899

ABSTRACT:
The invention concerns a probe for the detection of shigellae and entero-invasive E. coli, containing a nucleic acid sequence originating from the 140 Mdal virulence plasmid of the M 90 T strain of Shigella flexneri; having a maximum size of around 27 kb and inlcuding all or part of the 27 kb Bam HI fragment.
This probe permits the in vitro diagnosis of syndromes of dysentery or diarrhea, of the Shigellosis type.

REFERENCES:
patent: 4486539 (1984-12-01), Ranki et al.
patent: 4563419 (1986-01-01), Ranki et al.
Hale, T. L., et al, "Characterization of Virulence Plasmids . . . Shigella Flexver'. . . ", Inf. Smm. 40(1), 340-350 (Apr. 1983).
DNA Hybridization Technique to Detect `Shigella`, Species and Enteroinvasive Escherichia coli, C. R. Boileau, H. M. d'Hauteville, and P. J. Sansonetti, J. Clin. Microbiol. 20, No. 5, 959-961 (1984).
Hale et al, Chemical Abstracts, vol. 98, No. 19, p. 88, 155688c (May 9, 9, 1983).
Sansonetti et al, Chemical Abstracts, vol. 99, No. 25, p. 171, 207154t (Dec. 19, 1983).
Sansonetti et al, Colloque Inserm, vol. 114, pp. 537-548 (1983).
Sansonetti et al, Chemical Abstracts, vol. 96, No. 19, p. 440, 159173n (May 10, 1982).
Bao et al, Chemical Abstracts, vol. 100, No. 1, p. 116, 1322b (Jan. 2, 1984).

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