Probe for constructing probe-polymer method of constructing...

Details Probe for constructing probe-polymer method of constructing... Probe for constructing probe-polymer method of constructing...

2006-06-13

2006-06-13

Sitton, Jehanne (Department: 1634)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S023100, C435S006120, C435S091100

Reexamination Certificate

active

07060814

ABSTRACT:
The present invention provides a method for measuring a target gene under isothermal conditions without using enzyme. A pair of probes each having n (n≧3) base sequence regions complementary to each other are hybridized alternately to form a double-stranded probe-polymer. A base pair at branched sites of each complementary base sequence region is designed to be a G (guanine)-C (cytosine) bond, whereby a stable double-stranded probe-polymer is formed. One of complementary portions in one probe is constituted to have a base sequence complementary to a part of a target gene, whereby a target gene-probe-polymer complex is formed and the target gene is measured.

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