Probe containing a modified nucleic acid recognizable by specifi

Details Probe containing a modified nucleic acid recognizable by specifi Probe containing a modified nucleic acid recognizable by specifi

1990-04-24

1992-03-24

Yarbrough, Amelia Burgess

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 71, 435810, 436501, 436804, 436808, 436828, 5303891, 5303898, 935 78, 935 81, C12Q 168

Patent

active

050988255

ABSTRACT:
A method and kit for detecting a predetermined nucleotide sequence in a specimen using a nucleic acid probe modified with N-2-acetylaminofluorene (AAF).

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Grunberger et al., Coding and Conformational Properties of Oligonucleotides Modified with the Carcinogen N-2-Acetylaminofluorene, PNAS 66, 488-494 (1970).
Fuchs et al., Physical Studies on Deoxyribonucleic Acid after Covalent Binding of a Carcinogen, Biochemistry 11, 2659-2666 (1972).
Fuchs et al., Dynamic Structure of DNA Modified with the Carcinogen N-Acetoxy-N-2-acetylaminofluorene, Biochemistry 13, 4435-4440 (1974).
Fuchs, In Vitro Recognition of Carcinogen-Induced Local Denaturation Sites in Native DNA by S1 Endonuclease from Aspergillus Oryzae, Nature 257, 151-152 (1975).
Fuchs et al., Comparative Orientation of the Fluorene Residue in Native DNA Modified by N-Acetoxy-N-2-Acetylaminofluorene and Two 7-Halogeno Derivatives, Biochemistry 15, 3347-3351 (1976).
de Murcia, G. et al., Proc. Nat'l Acad. Sci., vol. 76, 1979, pp. 6076-6080.
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