Probe and a process for the detection of specific microorganisms

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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536 26, 536 27, 536 28, 935 77, 935 78, C12P 1934, C07H 1906, C07H 1512

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048106445

ABSTRACT:
The invention concerns a composition of fragments of DNA utilizable as a probe for the detection of specific micoorganism, notably Legionella, in environments which contain them. These compositions are obtained from the whole genomes of the microorganisms to be detected, after fragmentation of these genomes, particularly by using a restriction enzyme, and eliminating those sequences of the genomic DNA which are susceptible to being transcribed to ribosomal RNA.

REFERENCES:
patent: 4358535 (1982-11-01), Falkow et al.
van Ketel et al., Journ. Clin. Microbiol, 20 No. 3: 362-364 (1984).
Tompkins, L., Clinics in Laboratory Medicine, 5 No: 99-107, (1985).
Patamaroj, V. et al., Journ. Clin. Microbiol, 18, No. 6: 1429-1431 (1983).
Starr, M. et al., in "The Prokaryotes" vol. 1, (Stan et al. eds) Springer-Verlay Publishers pp. 3-42 (1981).
J. Ludwig et al., Chemical Abstracts, vol. 99, no. 15, p. 350, No. 118946k (Oct. 1983) & Arch. Microbiol., 135(1), pp. 45-50 (1983).
R. J. Van Ketel et al., Chemical Abstracts, vol. 101, No. 17, p. 363, No. 147436j (Oct. 22, 1984).
S. E. Hoffner et al., Chemical Abstracts, vol. 101, No. 15, p. 360, No. 126407k (Oct. 8, 1984) & Inst. Natl. Sante Rech. Med. (colloq.), 114, pp. 383-391 (1983).

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