Primers labeled with energy transfer coupled dyes for DNA sequen

Details Primers labeled with energy transfer coupled dyes for DNA sequen Primers labeled with energy transfer coupled dyes for DNA sequen

1995-03-27

1998-01-13

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 243, C12Q 168, C07H 2104

Patent

active

057078041

ABSTRACT:
Compositions are provided for making oligonucleotides carrying pairs of donor and acceptor dye molecules, designed for efficient excitation of the donor at a single wavelength and emission from the acceptor in each of the pairs at different wavelengths. The different molecules having different donor-acceptor pairs can be modified to have substantially the same mobility under separation conditions, by varying the distance between the donor and acceptor in a given pair, and to have enhanced emission intensities from the acceptor. Particularly, the fluorescent compositions find use as labels in sequencing nucleic acids.

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