Primers for the detection of spore forming bacteria

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 536 231, 536 243, 536 2432, 536 2433, C12Q 168, C12P 1934, C12N 1511, C07H 2104

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active

059288752

ABSTRACT:
A process using polymerize chain reaction (PCR) technology is described for the detection of spore forming bacteria in paper products and paper manufacturing streams and additives. Disclosed and claimed are novel nucleotide primers which specifically amplify sporulation genes common to spore forming bacteria. These primers produce gene fragments diagnostic for the presence of spore forming bacteria.

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Brill et al., "Differentiation Between Spore-Forming and Asporogenic Bacteria Using a PCR and Southern Hybridization Based Method," Journal of Microbiological Methods, (1997), vol. 31, pp. 29-36.

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