Primers for amplifying hsp 65 gene of mycobacterial species,...

Details Primers for amplifying hsp 65 gene of mycobacterial species,... Primers for amplifying hsp 65 gene of mycobacterial species,...

2003-01-21

2010-06-08

Swartz, Rodney P. (Department: 1645)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C536S022100, C536S023100, C536S023700, C536S024100, C536S024300, C536S024320, C435S004000, C435S006120

Reexamination Certificate

active

07732589

ABSTRACT:
The present invention relates to a pair of primers specific to mycobacterial species, a polynucleotide of an hsp 65 gene fragment, and a method for the identification of mycobacterial species by using the same. More specifically, the 604-bp hsp 65 gene fragment can be applied to identification methods ofmycobacteriasuch as the comparative sequence analysis method, the probe hybridization method, and PCR-RFLP, which can resolve the problems of a conventional identification method based on bio-chemical characteristics, where the genusmycobacteriumcovers various species and has a low growth rate, and of the problems of 16s rDNA. Thus, according to the identification method of the present invention, the mycobacterial species can be identified simply, economically, and accurately.

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