Primers for amplification of a genus specific sequence of the my

Details Primers for amplification of a genus specific sequence of the my Primers for amplification of a genus specific sequence of the my

1997-09-26

1999-11-16

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 435 912, C12Q 168, C07H 2102, C07H 2104, C12P 1934

Patent

active

059855690

ABSTRACT:
Disclosed herein are oligonucleotide primers and probes that can be used to detect species of medical interest in the genus Mycobacterium. Also disclosed are genus-specific methods and kits for detecting Mycobacterium using the disclosed oligonucleotide primers and probes. Preferably, Mycobacterium are detected by amplifying the Mycobactenium nucleic acids using the disclosed amplification primers, and then detecting the amplified nucleic acids. In a more preferred embodiment, Mycobacterium nucleic acids are amplified and detected by thermophilic strand displacement amplification.

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E. Avaniss-Aghajani, et al. "Molecular Technique for Rapid Identification of Mycobacteria" J. Clin. Microbiol. 34:98-102 (1996).
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