Primers and probes for detecting Pneumocystis carinii

Details Primers and probes for detecting Pneumocystis carinii Primers and probes for detecting Pneumocystis carinii

1995-05-23

1997-01-14

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, C12Q 168, C07H 2104

Patent

active

055938361

ABSTRACT:
Primers and probes can be used to detect nucleic acid from human strains of Pneumocystis carinii in a sample. The nucleotide sequence of a region of the 18S ribosomal RNA gene of a strain of P. carinii derived from humans is provided. The primers amplify regions of the 18S ribosomal RNA gene and hybridize to regions containing variability among strains. Probes hybridize to a regions within the amplified region that contain variability among strains.

REFERENCES:
Borensztein et al., Molecular and Cellular Probes 6:361-365 (1992).
Edman et al., 1988, "Ribosomal RNA Sequence Shows Pneumocystis carinii to be a Member of the Fungi" Nature 334: 519-522.
Lipschik et al., 1992, "Improved Diagnosis of Pneumocystis carinii Infection by Polymerase Chain Reaction on Induced Sputum and Blood" Lancet 340:203-206.
Medlin et al., 1988, "The Characterization of Enzymatically Amplified Eukaryotic 16S-like rRNA-Coding Regions" Gene 71:491-499.
Sogin and Edman, 1989, "A Self-Splicing Intron in the Small Subunit rRNA Gene of Pneumocystis carinii" Nucleic Acids Research 17(13): 5349-5359.
Wakefield et al., 1990, "Detection of Pneumocystis carinii With DNA Amplification" Lancet 336:451-453.
Wakefield et al., 1990, "Amplification of Mitochondrial Ribosomal RNA Sequences From Pneumocystis carinii DNA of Rat and Human Origin" Molecular and Biochemical Parasitology 43:69-76.
Kitada et al., 1991, "Diagnosis of Pneumocystis carinii Pneumonia by 5S Ribosomal DNA Amplification" J. Protozool. 38(6): 90S-91S.
Kitada et al., 1991, "Detection of Pneumocystis carinii Sequences by Polymerase Chain Reaction: Animal Models and Clinical Application to Noninvasive Specimens" J. Clinical Microbiology 29(9):1985-1990.
Sinclair et al., 1991, "Pneumocystis carinii Organisms Derived From Rat and Human Hosts are Genetically Distinct" Molecular and Biochemical Parasitology 45:183-184.
Cushion et al., 1993, "Evidence for Two Genetic Variants of Pneumocystis carinii Coinfecting Laboratory Rats" J. Clinical Microbiology 31(5):1217-1223.
Lee et al., 1993, "Nucleotide Sequence Variation in Pneumocystis carinii Strains That Infect Humans" J. Clinical Microbiology 31(3):754-757.
Stratagene Catalog (1988) p. 39.
GenBank Accession Nos. Q10826, Q10825, V10397, L19742 and L22158.

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