Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2007-07-24
2007-07-24
Kim, Young J. (Department: 1637)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C435S006120, C435S091100, C435S091200
Reexamination Certificate
active
10559062
ABSTRACT:
Oligonucleotide primers and polynucleotide probes provide selective amplification and detection of Id genetic sequences and are selected both (1) to provide the desired specificity so that they amplify only the specific Id type to which they are targeted; and (2) to introduce terminal restriction endonuclease cleavage sites into the amplicon that facilitate the incorporation of the amplicon into plasmid-based vectors. Detection of Id genetic sequences can be carried out using the labeled-amplicon by reamplifying the amplicon with the primers in the presence of labeled, for example radiolabeled, deoxynucleotide triphosphates. The probes of the invention correspond in sequence to the amplicons produced using the primers of the invention, after cleavage at the restriction endonuclease sites.
REFERENCES:
patent: 4530904 (1985-07-01), Hershberger et al.
Sequence Alignment.
Acession No. 395337 on electronic database, GenBank, Ellmeier et al., submission Nov. 18, 1993.
Accession No. 457784 on electronic database, GenBank, Deed et al., submission Jan. 19, 1995.
Haines et al., “Complex Conserved Organization of the Mammalian Leukemia Inhibitory Factor Gene: Regulated Expression of Intracellular and Extracellular Cytokines,” The Journal of Immunology, Apr. 1999, vol. 162, pp. 4637-4646).
Overdier et al., “The DNA-Binding Specificity of the Hepatocyte Nuclear Factor 3/forkhead Domain Is Influenced by Amino Acid Residues Adjacent to the Recognition Helix,” Molecular and Cellular Biology, Apr. 1994, vol. 14, No. 4, pp. 2755-2766.
Katagiri et al. Identification of a BMP-responsive element in Id1, the gene for inhibition of myogenesis. Genesis to cells, 2002, vol. 7, pp. 949-960.
Biggs et al. A human Id-like helix-loop-helix protein expressed during early development. PNAS. Feb. 1992, vol. 89, pp. 1512-1516.
Ellmeier et al. Mutually exclusive expression of a helix-loop-helix gene and N-mye in human neuroblastomas and in normal development. EMBO J, Jul. 1992, vol. 11, No. 7, Abstract only.
Kim Young J.
Marina Larson & Associates LLC
Sloan-Kettering Institute for Cancer Research
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