Primers and methods for simultaneous amplification of multiple m

Details Primers and methods for simultaneous amplification of multiple m Primers and methods for simultaneous amplification of multiple m

1995-05-01

1996-11-19

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 912, 536 243, C12Q 168, C12P 1934, C07H 2104

Patent

active

055761801

ABSTRACT:
The present invention relates to a DNA amplification primer pair for the simultaneous amplification of multiple highly polymorphic genomic loci, which comprises R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2). A method for the DNA fingerprinting identification of genetically related or unrelated individuals, which comprises the steps of: a) collecting genomic DNA sample of the individuals; b) performing DNA amplification of the DNA samples of step a) using the primer pair R14B264/Q560mak (SEQ ID NO:1/SEQ ID NO:2); and c) separating the amplified DNA segments of step b); whereby three markers of the genomic DNA of different size are amplified and serve as DNA fingerprinting of the individuals.

REFERENCES:
Sinnett, D. et al., 1988, Hum. Genet., 81:4-8.
Zietkiewicz, E. et al., 1992, Proc. Natl. Acad. Sci. USA., 89:8448-8451.
Tang et al., Mammalian Genome 6 :345-349 (1995).
The Stratagene Catalog (1988) p. 39.

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