Pretreatment of microprojectiles prior to using in a particle gu

Details Pretreatment of microprojectiles prior to using in a particle gu Pretreatment of microprojectiles prior to using in a particle gu

1993-11-24

1999-03-09

Achutamurthy, Ponnathapura

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

4351721, 435176, C12N 1500

Patent

active

058799184

ABSTRACT:
An improvement in the process of transporting biological materials into living cells by bombarding the cells with microprojectile particles coated with biological material. In the improved process prior to coating of the particles such as tungsten beads for example, with biological material such as DNA, the particles are first pretreated with a strong inorganic acid such as nitric acid.

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Sanford, "The Biolistic Process" Trends in Biotechnology, vol. 6 (1988), pp. 299-302.
Sanford, et al. "Delivery of Substances Into Cells and Tissue", Particle Science & Technology, vol. 5(1987), pp. 27-37.
"Applied Biosystems" (1986), DNA/RNA Extractor Notes DEN 340A/07 dated Dec. 18, 1986, pp. 1-4.

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