Pretreatment of microcarriers for cell culture

Details Pretreatment of microcarriers for cell culture Pretreatment of microcarriers for cell culture

1979-04-23

1980-12-02

Warden, Robert J.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

260 8, 435284, 435285, C08L 8900

Patent

active

042370337

ABSTRACT:
A method is provided for pretreatment of microcarrier beads to make them suitable for use in mass culture of vertebrate anchorage dependent cells such as primary and diploid cells. The beads are soaked and heated in fetal calf serum (FCS) at from about 65.degree. to about 95.degree. C. for about 3 minutes to about 1 hour.

REFERENCES:
patent: 3717551 (1973-02-01), Bizzini et al.
patent: 3850748 (1974-11-01), Cook et al.
patent: 3910819 (1975-10-01), Rembaum et al.
patent: 4036693 (1977-07-01), Levine et al.
A. L. van Wezel, "Microcarrier Cultures of Animal Cells," Tissue Culture Methods and Applications, Academic Press, pp. 372-377; 1973.
David W. Levine et al., "Microcarrier Cell Culture: New Methods for Research-Scale Application"" Somatic Cell Genetics, vol. 3, No. 2 pp. 149-155; 1977.
R. E. Spier et al., "The Production of Foot and Mouth Disease Virus from BHK 21C 13 Cells Grown on the Surface of DEAE Sephadex A50 Beads," Biotechnology and Bioengineering, vol. 18, pp. 659-667; 1976.

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