Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Patent
1983-11-04
1986-10-14
Marantz, Sidney
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
435 7, 435 18, 435 29, 435 38, 435871, 436533, 436534, 436548, 436511, C12Q 104
Patent
active
046172645
ABSTRACT:
The method disclosed herein allows for the pretreatment of organisms, suspected of being a material of interest, prior to performing an assay for a determination thereof. The method comprises contacting the organism in an aqueous medium with a composition comprising (1) an enzyme capable of hydrolyzing bonds between N-acetylglucosamine and N-acetylmuramic acid and (2) a chelating agent, in amounts and under conditions sufficient to produce a homogeneous suspension of the organism of interest in the aqueous medium but insufficient to produce lysed cells or spheroplasts. The method and composition are particularly applicable to the pretreatment of cells of gram negative bacteria such as, for example, N. gonorrhoeae, and of chlamydia.
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Barrett Sharon S.
Pritchard Cynthia G.
Whiteley Gordon R.
Barrett Carole F.
Herald William J.
Leitereg Theodore J.
Marantz Sidney
Syntex (U.S.A.) Inc.
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