Pretreatment method and composition

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435 7, 435 18, 435 29, 435 38, 435871, 436533, 436534, 436548, 436511, C12Q 104

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active

046172645

ABSTRACT:
The method disclosed herein allows for the pretreatment of organisms, suspected of being a material of interest, prior to performing an assay for a determination thereof. The method comprises contacting the organism in an aqueous medium with a composition comprising (1) an enzyme capable of hydrolyzing bonds between N-acetylglucosamine and N-acetylmuramic acid and (2) a chelating agent, in amounts and under conditions sufficient to produce a homogeneous suspension of the organism of interest in the aqueous medium but insufficient to produce lysed cells or spheroplasts. The method and composition are particularly applicable to the pretreatment of cells of gram negative bacteria such as, for example, N. gonorrhoeae, and of chlamydia.

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patent: 4281061 (1981-07-01), Zuk et al.
patent: 4443549 (1984-04-01), Sadowski
Audiffren et al., C. R. Societe De Biologie, (1967), vol. 161, No. 6, pp. 394-396.
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Caldwell et al., (1984), Infection and Immunity, vol. 44, No. 2, pp. 306-314.
Repaske, Biochimica et Biophysica Acta (1958), vol. 39, pp. 225-232.

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