Pretreating reagent, pretreatment method, assay with pretreated

Chemistry: analytical and immunological testing – Heterocyclic carbon compound – Hetero-o

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436 8, 436 63, 436 91, 436174, 436175, 435 4, G01N 3300

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active

053895479

ABSTRACT:
A reagent denatures or eliminates factors interfering with biochemical reactions by simple treatment without requiring separation of any denatured product precipitate. The reagent makes it possible to assay, in particular, .beta.-glucan and endotoxin in blood-derived samples rapidly and efficiently with high sensitivity. The reagent includes a hexadimethrine compound and an alkali metal hydroxide or an alkali metal hydroxide as a main component. A method for assaying a substance specifically reacting with a Limulus reagent utilizing the reagent, an assay kit including at least the reagent and a Limulus reagent, and a method of diagnosing infectious diseases based on the results obtained by the assay method are also provided.

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K. Inada et al., Establishment of a New Perchloric Acid Treatment Method to Allow Determination of the Total Endotoxin Content in Human Plasma by the Limulus Test and Clinical Application, Microbiol. Immunol., vol. 35, No. 4, pp. 303-314, 1991.
T. Obayashi et al., A new Chromogenic Endotoxin-Specific Assay Using Recombined Limulus Coagulation Enzymes and its Clinical Applications, Clinica Chimica Acta, vol. 149, No. 1, pp. 55-65, Jun. 1985.
H. Tamura et al., A New Sensitive Microplate Assay of Plasma Endotoxin, Journal of Clinical Laboratory Analysis, vol. 6, No. 4, pp. 232-238, 1992.

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