Presynaptic protein cast

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues

Reexamination Certificate

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Details

C435S069100, C435S320100, C435S325000, C536S023100, C536S023500

Reexamination Certificate

active

10747065

ABSTRACT:
The present invention enabled the detection and quantification of CAST, which is localized to synapses and tightly bound to the cytomatrix, and of the mRNA encoding the CAST. Furthermore, it was revealed that CAST functions as a protein scaffold for localizing RIM1 to synapses, contributing as a molecular basis for active zone formation.

REFERENCES:
patent: 2004/0029790 (2004-02-01), Patturajan et al.
patent: WO 01/75067 (2001-10-01), None
patent: WO 01/75067 (2001-10-01), None
patent: WO 02/070539 (2002-09-01), None
patent: WO 02/070539 (2002-09-01), None
Database Genesequence Online, Accession No. ABG05140, Drmanac, R.T., et al., Entry Date Feb. 2002.
Database Genesequence Online, Accession No. AAS69327, Drmanac, R.T., et al., Entry Date Feb. 2002.
Ko, J., et al., “Interaction of the ERC Family of RIM-binding Proteins with the Liprin-α Family of Multidomain Proteins,”J. Biol. Chem. 278:42377-42385, American Society for Biochemistry and Molecular Biology, Inc. (Oct. 2003).
Nagase, T., et al., “Prediction of the Coding Sequences of Unidentified Human Genes. VII. The Complete Sequences of 100 New cDNA Clones from Brain Which Can Code for Large Proteins in vitro,”DNA Res. 4;141-150, Kazusa DNA Research Institute and Universal Academy Press (1997).
Ohtsuka, T., et al., “CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and Munc13-1,”J. Cell. Biol. 158:577-590, Rockefeller University Press (Aug. 2002).
Ohtsuka, T., “Functional Protein-Protein Interactions at the Presynaptic Active Zone,” Abstract and Presentation Slides for a RIKEN Brain Science Institute Seminar, KAN Research Institute, pp. 1-11 (Aug. 26, 2002).
Okazaki, N., et al., “Prediction of the Coding Sequences of Mouse Homologues of KIAA Gene: II. The Complete Nucleotide Sequences of 400 Mouse KIAA-homologous cDNAs Identified by Screening of Terminal Sequences of cDNA Clones Randomly Sampled from Size-fractionated Libraries,”DNA Res. 10:35-48, Kazusa DNA Research Institute And Universal Academy Press (Feb. 2003).
Wang, Y., et al., “A family of RIM-binding proteins regulated by alternative splicing: Implications for the genesis of synaptic active zones,”Proc. Natl. Acad. Sci. USA 99:14464-14469, National Academy of Sciences (Oct. 2002).
Schoch, S., et al., “RIM1α forms a protein scaffold for regulating neurotransmitter release at the active zone,”Nature 415:321-326, Nature Publishing Group (Jan. 2002).
Reeck, G.R., et al., “Homology In Proteins and Nucleic Acids: A Terminology Muddle and a Way Out of It,”Cell 50:667, Cell Press (1987).

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