Pressure-enhanced extraction and purification

Details Pressure-enhanced extraction and purification Pressure-enhanced extraction and purification

1998-05-22

2000-09-19

Wilson, James O.

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

Including freezing; composition therefor

435 72, 435 721, 435 722, 435 731, 435 732, A01N 100

Patent

active

061209856

ABSTRACT:
Methods for cell lysis and purification of biological materials, involving subjecting a sample maintained at a subzero temperature to high pressure, are disclosed. Apparatus for practicing the methods are also disclosed. The cell or cells that are lysed may be in suspension or part of a tissue. They are lysed by a method that includes: (i) providing a frozen cell or cells under atmospheric pressure; (ii) while maintaining the cell or cells at a subzero temperature, exposing the cell or cells to an elevated pressure in a pressure chamber, the elevated pressure being sufficient to thaw the frozen cell or cells at the subzero temperature; (iii) depressurizing the pressure chamber to freeze the cell or cells at the subzero temperature; and (iv) repeating the exposing and depressurizing steps until the cell or cells are lysed. This method can lyse a cell or cells with or without cell walls; such cells include, but are not limited to, bacteria, viruses, fungal cells (e.g, yeast cells), plant cells (e.g, corn leaf tissue), animal cells, insect cells, and protozoan cells.

REFERENCES:
patent: 4323439 (1982-04-01), O'Farrell
patent: 4559298 (1985-12-01), Fahy
patent: 5346999 (1994-09-01), Cathcart et al.
patent: 5605839 (1997-02-01), Simpson et al.
Mozhaev et al., "Exploiting the Effects of High Hydrostatic Pressure in Biotechnological Applications", TIBTECH, vol. 12, pp. 493-501, (1994).

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