Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2007-01-31
2011-10-04
Kim, Alexander (Department: 1656)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C702S027000
Reexamination Certificate
active
08030449
ABSTRACT:
Preparation of cryocooled protein crystal is provided by use of helium pressurizing and cryocooling to obtain cryocooled protein crystal allowing collection of high resolution data and by heavier noble gas (krypton or xenon) binding followed by helium pressurizing and cryocooling to obtain cryocooled protein crystal for collection of high resolution data and SAD phasing simultaneously. The helium pressurizing is carried out on crystal coated to prevent dehydration or on crystal grown in aqueous solution in a capillary.
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Kim, Chae-Un, et al., “High-Pressure Cooling of Protein Crystals Without Cryoprotectants”, Acta Cryst. D61, 881-890 (2005).
Kim, Chae-Un, et al., “Solution of Protein Crystallographic Structures by High-Pressure Cryocooling and Noble-Gas Phasing”, Acta Cryst. D62, 687-694 (2006).
Albright, Ronald A., et al., “The RCK Domain of the KtrAB K+Transporter: Multiple Conformations of an Octameric Ring”, Cell 126, 1147-1159 Sep. 22, 2006.
Gruner Sol M.
Kim Chae Un
Cornell Research Foundation Inc.
Hodgson & Russ LLP
Kim Alexander
LandOfFree
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