Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or...
Patent
1991-02-17
1995-12-26
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Differentiated tissue or organ other than blood, per se, or...
435 2, 435 71, 435 72, 435 721, 4352401, 435219, C12N 950, A01N 102, C12Q 100, G01N 3353
Patent
active
054787220
ABSTRACT:
Cells are treated to preserve them in non-frozen hydrated form with minimal alterations to their antigens or nucleic acids. This makes the cells useful as quality control (QC) reagents for processes such as calibrating and standardizing flow cytometry equipment, for use as "unknown" test samples for QC testing programs, and to prepare patient specimens for archival storage. This method includes: (1) treating the cells to inhibit proteolysis, preferably using several protease inhibitors; (2) fixing the cells, either by contacting them with a crosslinking agent such as formalin or paraformaldehyde, or by exposure to microwave radiation, while the cells are being agitated to promote the formation of crosslinking bonds within a single cell while inhibiting the formation of cell clumps; and (3) if the cells are treated with a chemical crosslinking agent, removing the crosslinking agent and contacting the cells with a quenching agent. The cells should be stored in a solution containing protease inhibitors. This method causes little or no detectable alteration of antigenic or DNA histograms. It has been used to preserve cells that have been stored for months at room temperature, or for more than a year at refrigerated temperatures, with no substantial deterioration of surface or cytoplasmic antigens or DNA content.
REFERENCES:
patent: 3682776 (1972-08-01), Grundmann et al.
patent: 4591572 (1986-05-01), Mattes et al.
patent: 4857451 (1989-08-01), Schwartz
D. Levitt & M. King, "Methanol Fixation . . . " Journal of Immunological Methods 96: 233-237 (1987).
R. W. Schroff et al, "Detection of Intra-Cytoplasmic Antigens . . . " Journal of Immunological Methods 70:167-177 (1984).
L. Harvath, "Quality control in clinical flow cytometry," Pathology and Immunopathology Research 7: 338-344 (1988).
P. K. Horan et al, "Standards and Controls in Flow Cytometry," chapter 21 (pp. 397-414) in Melamed et al, eds., Flow Cytometry and Sorting, 2nd edition, Wiley & Liss, New York (1990).
A. Feldman and R. W. Dapson, "The Chemistry of Fixation and Staining, Part I: Basic Concepts", J. Histotechnology 3(4): 132-135 (1980).
R. W. Dapson and A. Feldman, "The Chemistry of Fixation and Staining, Park II: A Unified Theory of Acid/Base Staining Reactions," J. Histotechnology 3(4): 138-142 (1980).
C. W. Caldwell et al, "Fluorescence Intensity as a Quality Control Parameter in Clinical Flow Cytometry," Amer. J. Clinical Pathology 88(4): 447-456 (1987).
Sales literature on Cyto-Trol.TM. lyophilized cells, distributed by Coulter Company.
Babcock, G. F., et al, "Use of Previously Fixed Lymphocytes for Immunophenotyping," Abstract #544B, Cytometry, Suppl. 5 (p. 102), 1991.
Lal, R. B., et al, "Fixation and Long-term Storage of Human Lymphocytes for Surface Market Analysis by Flow Cytometry," Cytometry 9: 213-219 (1988).
Lankford L. Blaine
Robinson Douglas W.
The Curators of the University of Missouri
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