Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1994-04-19
1998-07-07
Lankford, Blaine
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435 11, 435 2, 435 721, 435219, 436 1, 436 8, 436 15, 436 16, C12N 506, C12N 502, A01N 100
Patent
active
057767548
ABSTRACT:
This invention discloses a population of cells which have been preserved in non-frozen hydrated form, and which have been chemically treated in a manner that renders them metabolically inert and free of proteolytic enzyme activity, but without significantly altering the cell surface proteins that are of interest in flow cytometry. The absence of alteration of the surface proteins is indicated by the ability of the surface proteins to bind to monoclonal antibodies that bind to untreated proteins of the same type, with approximately the same affinity as proteins on untreated cells. The preserved cells and their surface antigens are stable for at least a month when stored at 4.degree. C. in buffered saline solution. These cell preparations are useful as quality control (QC) reagents for processes such as calibrating and standardizing flow cytometry equipment, and for use as "unknown" test samples for QC testing programs, or as patient specimens for archival storage and subsequent retrospective analysis.
REFERENCES:
patent: 3682776 (1972-08-01), Grundmann et al.
Lal et al. Cytometry. 9: 213-219, 1988.
Lankford Blaine
The Curators of the University of Missouri
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