Preservation solution for ex vivo, warm preservation of tissues,

Details Preservation solution for ex vivo, warm preservation of tissues, Preservation solution for ex vivo, warm preservation of tissues,

1995-01-13

1997-02-04

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

435 12, 435405, 435406, A01N 102, C12N 500

Patent

active

055996590

ABSTRACT:
The present invention is directed to a new hyperosmolar preservation solution useful in supporting the simultaneous in vitro growth of, and preservation of vascular endothelial cells from large vessel and microvessel origins. In addition, the preservation solution of the present invention may be used for initial flushing, and as a perfusate for storage of organs intended for transplantation using a warm preservation technology at between 18.degree. C. to 35.degree. C. Among the components of the preservation solution are colloid, mucopolysaccharide, retinal-derived fibroblast growth factor and a high magnesium concentration. Also, the present invention is directed to a method for preserving, without extreme hypothermia, an organ intended to be transplanted using the preservation solution.

REFERENCES:
patent: 5455162 (1995-10-01), Bellamy et al.
Nakaya S et al., Proceeding Xth Int. Con. Nutrition: Sym. Pefluorochem. Art. Blood, Kyoto pp. 187-201 (1975).
Lass J H et al, Refrac & Corneal Surg. 6:92-98 (1990).
Meyer D R et al., J. Toxicol-Cut. & Ocular Toxicol 10(1&2):59-77 (1991).
Okamoto R et al., Transplant Proc 20 (1):969-71 (1988).

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