Preservation of RNA in a biological sample

Details Preservation of RNA in a biological sample Preservation of RNA in a biological sample

2006-06-06

2006-06-06

Whisenant, Ethan (Department: 1634)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C536S023100, C536S024300

Reexamination Certificate

active

07056673

ABSTRACT:
To preserve RNA in a biological sample for analysis, the sample is incubated with an RNA preservative capable of precipitating RNA in an aqueous solution, such as a triphenylmethane dye (e.g., methyl green, crystal violet, pararosaniline, or tris-(4-aminophenyl)methane), cresyl violet, or cobalt ions. RNA preservation may be used in an immunostaining assay and other histochemical methods.

REFERENCES:
patent: 4943531 (1990-07-01), Goff et al.
patent: 5242974 (1993-09-01), Holmes
patent: 5322770 (1994-06-01), Gelfand
patent: 5384261 (1995-01-01), Winkler et al.
patent: 5405776 (1995-04-01), Kotewicz et al.
patent: 5405783 (1995-04-01), Pirrung et al.
patent: 5412087 (1995-05-01), McGall et al.
patent: 5424186 (1995-06-01), Fodor et al.
patent: 5429807 (1995-07-01), Matson et al.
patent: 5436327 (1995-07-01), Southern et al.
patent: 5445934 (1995-08-01), Fodor et al.
patent: 5472672 (1995-12-01), Brennan
patent: 5527681 (1996-06-01), Holmes
patent: 5529756 (1996-06-01), Brennan
patent: 5532128 (1996-07-01), Eggers et al.
patent: 5545531 (1996-08-01), Rava et al.
patent: 5554501 (1996-09-01), Coassin et al.
patent: 5556752 (1996-09-01), Lockhart et al.
patent: 5561071 (1996-10-01), Hollenberg et al.
patent: 5571639 (1996-11-01), Hubbell et al.
patent: 5593839 (1997-01-01), Hubbell et al.
patent: 5599695 (1997-02-01), Pease et al.
patent: 5624711 (1997-04-01), Sundberg et al.
patent: 5658734 (1997-08-01), Brock et al.
patent: 5700637 (1997-12-01), Southern
patent: 5807522 (1998-09-01), Brown et al.
patent: 6004755 (1999-12-01), Wang
patent: 6204375 (2001-03-01), Lader
patent: 6420132 (2002-07-01), Bonner et al.
patent: 2002/0009768 (2002-01-01), Chu
patent: 2002/0102666 (2002-08-01), Reiter et al.
patent: 2002/0177149 (2002-11-01), Rimm et al.
patent: 2002/0197637 (2002-12-01), Wilson et al.
patent: 2003/0152928 (2003-08-01), Asai et al.
patent: WO9521944 (1995-08-01), None
Adams, “Binding Of Crystal Violet By Nucleic Acids ofEscherichia coli”, J. Pharm. Pharmacol., vol. 20, Suppl: pp. 18S-22S (1968).
Amann et al., “Identification In Situ And Phylogeny Of Uncultured Bacterial Endosymbionts”,Nature, vol. 351, pp. 161-164 (1991).
Armstrong et al., “A Comparison Of The Interaction Of An Acridine Dye And A Triphenylmethane Dye With Deoxyribonucleic Acid”,J. Am. Chem. Soc., vol. 94(22), pp. 7650-7653 (1972).
Boonacker et al., “Enzyme Cytochemical Techniques For Metabolic Mapping In Living Cells, With Special Reference To Proteolysis”,J. Histochem. Cytochem., vol. 49(12), pp. 1473-1486 (2001).
Danguy, “Perspectives In Modern Glycohistochemistry”,Eur. J. Histochem., vol. 39, pp. 5-14 (1995).
Eltoum et al., “Microdissection Of Histologic Sections: Past, Present, And Future”,Adv. Anat. Pathol., vol. 9(5), pp. 316-322 (2002).
Emmert-Buck et al., “Laser Capture Microdissection”,Science, vol. 274, pp. 998-1001 (1996).
Fend et al., “Immuno-LCM: Laser Capture Microdissection Of Immunostained Frozen Sections For mRNA Analysis”,Am. J. Pathol., vol. 154(1), pp. 61-66 (1999).
Fink et al., “Immunostaining And Laser-Assisted Cell Picking For mRNA Analysis,”Lab. Invest., vol. 80(3), pp. 327-333 (2000).
Fox et al., “Methyl Green And Its Analogues Bind Selectively To AT-Rich Regions Of Native DNA”,Eur. J. Histochem., vol. 36, pp. 263-270 (1992).
Goldsworthy et al., “Effects Of Fixation On RNA Extraction And Amplification From Laser Capture Microdissected Tissue”,Mol. Carcinog., vol. 25, pp. 86-91 (1999).
Graeber et al., “Axotomy Of The Rat Facial Nerve Leads To Increased CR3 Complement Receptor Expression By Activated Microglial Cells”,J. Neurosci. Res., vol. 21, pp. 18-24 (1988).
Jiang et al., “Use Of Interphase Fluorescence In Situ Hybridization As A Powerful Diagnostic Tool In Cytology”,Diagn. Mol. Pathol., vol. 11(1), pp. 47-57 (2002).
Kim et al., “Methyl Green. A DNA Major-Groove Binding Drug”,FEBS Lett., vol. 315(1), pp. 61-64 (1993).
Kohda et al., “Analysis Of Segmental Renal Gene Expression By Laser Capture Microdissection”,Kidney Int., vol. 57, pp. 321-331 (2000).
Krizman et al., “Construction Of A Representative cDNA Library From Prostatic Intraepithelial Neoplasia”,Cancer Res., vol. 56, pp. 5380-5383 (1996).
Leethanakul et al., “Distinct Pattern Of Expression Of Differentiation And Growth-Related Genes In Squamous Cell Carcinomas Of The Head And Neck Revealed By The Use Of Laser Capture Microdissection And cDNA Arrays”,Oncogene, vol. 19, pp. 3220-3224 (2000).
McNicol et al., “In Situ Hybridization And Its Diagnostic Applications In Pathology”,J. Pathol., vol. 182, pp. 250-261 (1997).
Melnick et al., “The Mechanism Of The Drug Induced Partial Displacement Of Methyl Green From DNA”,Biochem Int., vol. 16, pp. 69-75 (1988).
Muller et al., “Interactions Of Heteroaromatic Compounds With Nucleic Acids. A-T-Specific Non-Intercalating DNA Ligands”,Eur. J. Biochem., vol. 54, pp. 385-394 (1975).
Murakami et al., “IF-LCM: Laser Capture Microdissection Of Immunofluorescently Defined Cells For mRNA Analysis”,Kidney Int., vol. 58, pp. 1346-1353 (2000).
Okuducu et al., “Influence Of Histochemical Stains On Quantitative Gene Expression Analysis After Laser-Assisted Microdissection”,Int. J. Mol. Med., vol 11, pp. 449-453 (2003).
Pabon et al., “Optimized T7 Amplification System For Microarray Analysis”,Biotechniques, vol. 31(4), pp. 874-879 (2001).
Quan et al., “Induction Of Inhibitory Factor KappaBalpha mRNA In The Central Nervous System After Peripheral Lipopolysaccharide Administration: An In Situ Hybridization Histochemistry Study In The Rat”,Proc. Natl. Acad. Sci. USA, vol. 94, pp. 10985-10990 (1997).
Sagar et al., “Expression Of c-Fos Protein In Brain: Metabolic Mapping At The Cellular Level”,Science, vol. 240, pp. 1328-1331 (1988).
Schutze et al., “Identification Of Expressed Genes By Laser-Mediated Manipulation Of Single Cells”,Nat. Biotechnol., vol. 16, pp. 737-742 (1998).
Smith et al., “Fluorescence Detection In Automated DNA Sequence Analysis”,Nature, vol. 321, pp. 674-679 (1986).
Sovago et al., “An Overview On Functional Receptor Autoradiography Using [35S]GTPgammaS”,Brain Res. Rev., vol. 38, pp. 149-164 (2001).
Streit, An Improved Staining Method For Rat Microglial Cells Using The Lectin FromGriffonia simplicifolia(GSA I-B4),J. Histochem. Cytochem., vol. 38(11), pp. 1683-1686 (1990).
Swanson, “Foundations Of Immunohistochemistry. A Practical Review”,Am. J. Clin. Pathol., vol. 90(3), pp. 333-339 (1988).
Vijayanathan et al., “DNA Condensation By Polyamines: A Laser Light Scattering Study Of Structural Effects”,Biochemistry, vol. 40, pp. 13644-13651 (2001).
Vocke et al., “Analysis Of 99 Microdissected Prostate Carcinomas Reveals A High Frequency Of Allelic Loss On Chromosome 8p12-21”,Cancer Res., vol. 56(10), pp. 2411-2416 (1996).
Yang et al., “Design Issues For cDNA Microarray Experiments”,Nature Reviews, vol. 3, pp. 579-588 (2002).

Affiliated with

Also associated with

Rating

Preservation of RNA in a biological sample does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Preservation of RNA in a biological sample, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Preservation of RNA in a biological sample will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3703000