Preservation of RNA and morphology in cells and tissues

Details Preservation of RNA and morphology in cells and tissues Preservation of RNA and morphology in cells and tissues

2006-11-21

2006-11-21

Lankford, Jr., Leon B. (Department: 1651)

Chemistry: molecular biology and microbiology

Differentiated tissue or organ other than blood, per se, or...

C435S374000, C436S018000

Reexamination Certificate

active

07138226

ABSTRACT:
A solution for preservation and/or storage of a cell or tissue is described. This simple nonaqueous composition can have 10% polyethylene glycol and 90% methanol. It can be used at room temperature. Special chemicals, equipment, and techniques are not needed. Tissue preserved with and/or stored in the solution can be processed for cytology or histology, including chemical staining and/or antibody binding, by a variety of methods; antigen, DNA, and RNA can be extracted from processed tissue in high yield and with minimal or no degradation. Advantages of the solution include: economy and safety, easy access to archival material, and compatibility with both cellular and genetic analyses. The use and manufacture of the solution are also described.

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