Preservation by cold storage

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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4352404, 435243, 435250, 4352521, 435254, 435255, 435256, 435260, C12N 100, C12N 500, C12N 104, C12N 130

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active

048638650

ABSTRACT:
Material which contains water, or is accompanied by an aqueous phase, notably biological cells, cell components or cell aggregates, or differentiated biological tissue is preserved by dispersion in an oil medium and under-cooling the dispersion, preferably to a temperature in the range -20.degree. C. to -40.degree. C. The oil medium is characterized by the absence of surfactant which can catalyze ice formation and is an immobile gel at the storage temperature. The preferred oil 10 medium is paraffin oil, or oil plus paraffin wax.

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Kline et al., "New Technique of Kidney Tissue Processing for Immunoflourescence Microscopy: Formol Sucrose/Gum Sucrose/Paraffin", Laboratory Investigation, vol. 47(2), (1982) pp. 206-213.
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Mathias et al., "Nucleation and Growth of Ice in Deeply Undercooled Erythrocytes", Cryobiology, vol. 21 (1984), pp. 123-132.

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