Prepressor protein gene for regulating expression of polypeptide

Details Prepressor protein gene for regulating expression of polypeptide Prepressor protein gene for regulating expression of polypeptide

1990-03-30

1993-05-11

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435143, 43525233, 4353201, 536 232, C12N 1500, C12N 1554, C12N 1570

Patent

active

052100251

ABSTRACT:
A nucleotide sequence coding for a repressor protein for regulating gene expression comprises about a 687 bp nucleotide region beginning about 81 bases upstream from the 2,2-dialkylglycine decarboxylase structural gene shown in FIG. 3. The repressor protein comprises about 229 amino acids. The nucleotide sequence is useful for regulating gene expression in recombinant expression vectors. The vectors and E. coli cells transformed with the vectors are useful for preparing Pseudomonas cepacia 2,2-dialkylglycine decarboxylase.

REFERENCES:
Sato, S. et al., 1978, Agricultural and Biological Chemistry (Tokyo), 42: 2341-2346.
Aaslestad, H. G. and A. P. Larson, 1964, Journal of Bacteriology, 88: 1296-1303.
Keller, J. W. et al., 1988, FASEB Journal 2(5): Abstract 6111 (Abstract for FASEB Annual Meeting, May 1-5, 1988).
Keller, J. W. et al., 1989, Journal of Cell Biology 107 (6 punt 3): (Abstract 2276) 399A (Abstract for Joint Meeting Am. Soc. Cell Biol. and Am. Soc. Biochem Med. Biol. 1989).
Keller et al., Pseudomonas cepacia 2,2-Dialkylglycine Decarboxylase-Sequence and Expression in Escherichia coli of Structural and Repressor Genes, J. Biol. Chem. 265(10):5531-5539 (1990).

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